品系特点
同源的
  
靶向突变
  
捐赠者 IMR Colony - 杰克森实验室
  
原始参考文献
Info 如果在研究中使用了Rag1敲除小鼠品系,请在文章发表时引用原始文献,并在材料与方法部分提及JAX品系号002216。
查阅参考文献 >
  
品系详情
此品系为 Rag1tm1Mom 突变纯合小鼠,不会产生成熟 T 细胞或成熟 B 细胞。Prkdcscid 纯合小鼠可以产生一些 B 细胞和 IgM(即,“渗漏”),Rag1tm1Mom 纯合小鼠则缺乏所有类型的成熟淋巴细胞(即,“无渗漏”)。Rag1 敲除小鼠没有 CD3+ 或 T 细胞受体 (T cell receptor, TCR) αβ阳性细胞。杂合子或野生型同窝小鼠胸腺中细胞含量是突变小鼠的 15 到 130 倍。胸腺细胞为 CD8阴性CD4阴性且大多为 IL2受体阳性。脾脏及骨髓中均不含 IgM 或 IgD 染色阳性的细胞,指示成熟 B 细胞完全缺失。这些数据和其他数据表明,B 细胞和 T 细胞发育在早期已经被阻断。通过肉眼观察难以区分该突变体与杂合子或正常野生型同胞小鼠。
品系建立
Rag1tm1Mom 突变品系由麻省理工学院癌症研究中心 Susumu Tonegawa 博士实验室的 Peter Mombaerts 博士建立。在品系建立过程中,使用了带 Pgk-neo 标记的靶向载体。该靶向载体引起的同源重组导致编码序列 5' 端有 1356 bp 长度的缺失。使用的是源自 129S7/SvEvBrd-Hprt+ 的 AB1 胚胎干细胞系。 将携带 Rag1tm1Mom 突变的小鼠与 C57BL/6J 近交小鼠回交十代,即获得背景为C57BL/6J的品系。
参考文献
精选参考文献
如果在研究中使用了Rag1敲除小鼠品系,请在文章发表时引用原始文献,并在材料与方法部分提及JAX品系号002216。

1992

RAG-1-deficient mice have no mature B and T lymphocytes.

Mombaerts P , et al.

PubMed:1547488

MGI:J:1934

Cell 68(5):869-77

其它参考文献
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PubMed:15265889
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Shresta S , et al.

PubMed:14990690
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2004

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An important regulatory role for CD4+CD8 alpha alpha T cells in the intestinal epithelial layer in the prevention of inflammatory bowel disease.

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2003

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2003

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2002

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2001

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2001

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PubMed:11313258
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2001

Defective development of NK1.1(+) T-cell antigen receptor alphabeta(+) cells in zeta-associated protein 70 null mice with an accumulation of NK1.1(+) CD3(-) NK-like cells in the thymus.

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2001

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Chronic murine colitis is dependent on the CD154/CD40 pathway and can Be attenuated by anti-CD154 administration

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Impaired control of Brucella melitensis infection in Rag1-deficient mice.

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1992

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等位基因参考文献
基因分型和繁育

近似对照

Allele Symbol Rag1<tm1Mom>
Allele Specific Protocols
  
Rag1tm1Mom品系通过纯合子配繁建立种群。该品系需要饲养在与Prkdcscid纯合小鼠或其它免疫缺陷品系相同的无特定病原体(SPF)环境下。仅能订购纯合小鼠。
繁育策略
纯合子X纯合子
外观
详情

 

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Related Genotype: a/a

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002216
Rag1 KO
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